Journal: The Journal of investigative dermatology
Article Title: Regulation of Human Skin Pigmentation in situ by Repetitive UV Exposure - Molecular Characterization of Responses to UVA and/or UVB
doi: 10.1038/jid.2010.5
Figure Lengend Snippet: Immunohistochemistry of melanocyte-specific proteins encoded by UV-regulated genes. Pmel17, TYR, DCT and MITF were identified by staining with α PEP13h, α PEP7h, α PEP8 and Ab3, respectively, using Texas red. All specimens were from subject 2 and all are at the same magnification (200X); insets show regions depicted by the dashed boxes at 600X magnification. Numbers represent quantitation (mean ± SEM) of staining density in 6 different images each from 1 biopsy from each of the 4 areas on each of the 6 subjects (n=6 specimens, 36 images for each area). * = p <0.05. Bar = 50 μm.
Article Snippet: Melanosomal proteins were detected using the following primary antibodies: anti-human MITF mouse monoclonal Ab3 (1:4,000 dilution; NeoMarkers, Fremont, CA) and anti-human MART-1 mouse monoclonal Ab-3 (1:200 dilution; NeoMarkers), polyclonal rabbitα PEP7h for human TYR (1:8,000 dilution), polyclonal rabbit α PEP8h for human DCT (1:7,500 dilution) and polyclonal rabbit α PEP13hfor human Pmel17 (1:4,000 dilution) ( Virador et al. , 2001 ).
Techniques: Immunohistochemistry, Staining, Quantitation Assay